8/6/2023 0 Comments Non cyclic amp moleculeNot for human or animal therapeutic or diagnostic use.Glaucoma is characterized by a slow and progressive degeneration of the optic nerve, including retinal ganglion cell (RGC) axons in the optic nerve head (ONH), leading to visual impairment. Many different cell types have been grown successfully on the precoated microplates, which also have a clear bottom to allow monitoring of cells.įor Research Use Only. This provides better assay precision with one less transfer step. This cAMP-Screen Direct™ system has all of the same benefits as the cAMP-Screen™ system, but also eliminates the need to transfer cell lysates from a tissue culture plate to the precoated microplates because the cells can be grown directly in the microplates. After washing to remove unbound cAMP-AP, the chemiluminescent substrate is added, and the resulting glow signal is measured in a luminometer. In the presence of cAMP, the amount of cAMP-AP conjugate captured decreases as a result of competition for binding with unlabeled cAMP, causing a reduced signal signal reduction is proportional to the amount of cAMP present in the cell lysate. In samples without cAMP, all of the cAMP-AP conjugate is captured on the coated surface, resulting in a high signal. Lysates are incubated with a cAMP-AP conjugate and an anti-cAMP antibody in a coated microplate the resulting immune complexes are captured in the plate. Cell lysates are prepared in either the presence or absence of culture media. Cells are seeded into plates, cultured, and treated with test compounds as desired. The assay can be used for high-throughput screens for compounds that stimulate or interfere with these signal transduction pathways.Ī Simple Protocol-No Lysate Transfer Required In addition it has been used for receptor characterization, orphan receptor ligand identification, and the characterization of novel chimeric receptors. The assay has been used with established cell lines for functional measurements with endogenous receptors, cell lines with exogenously expressed ligand receptors on the cell surface, primary cell cultures, and tissues in response to treatment with the appropriate ligands. The cAMP-Screen Direct™ system is designed for quantitation of cellular cAMP for functional assays of receptor activation. In addition, the assay exhibits exceptionally low cross-reactivity with other adenosine-containing or cyclic nucleotides. This is useful in screens where several plates are compared with each other. Once the substrate/enhancer formulation reaches glow signal, the plate can be read for hours with little or no degradation of the signal. Intra-assay precision for duplicate samples is typically 5% or less. This is especially helpful in cell-based assays when measuring Gs- or Gi-coupled agonist or antagonist stimulation and/or inhibition. The assay has a wide dynamic range, detecting from 0.06 to 6,000 picomoles of cAMP without the need for sample dilution or manipulations such as acetylation. As few as 60 femtomoles of cAMP can be detected. This chemiluminescent assay is designed to provide the highest sensitivity of any commercially available cAMP assay. High Sensitivity and Hours of Steady Glow Time Useful for a wide range of receptor activation studies.A simple protocol with cells grown directly on the assay plate.Hours of read time with little or no degradation of the signal.The highest sensitivity of any commercially available cAMP assay.This assay is mainly used in secondary screening and pre-clinical research, where sensitivity and no false positives are essential. The chemiluminescent substrate/enhancer formulation is a ready-to-use reagent that generates a sustained-glow light emission from 30 minutes after addition. The cAMP assay utilizes the highly sensitive chemiluminescent CSPD™ Substrate with Sapphire-II™ Enhancer that is triggered by an enzyme conjugate composed of cAMP bound to alkaline phosphatase (cAMP-AP). This competitive chemiluminescent immunoassay is formatted with maximum flexibility to permit either manual reagent additions or automated high-throughput processing. The cAMP-Screen Direct™ 96-well Cyclic AMP Immunoassay System enables ultrasensitive determination of cyclic AMP (cAMP) levels in cell lysates.
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8/6/2023 0 Comments The next paradigm shiftOur infrastructure is changing and what this means is that providers can potentially slow down their costs for hardware upgrades by making the existing infrastructure more efficient which, in turn, means that consumers may also see a slower increase of cost over time as well. This potentially a game changer and something to watch for the future. Microsoft is now muscling into the game in a major way with the support of a major new development community. Traditionally Linux has dominated this arena. This, in and of itself, is also a paradigm shift in infrastructure thinking. Microsoft and Docker recently have recently announced a collaborative effort to natively integrate Docker into the new Windows Server 2016 platform. Previously I had written about Microsoft’s new love affair with Linux. These companies are now offering their own mainstream containerization systems and advertising them to the IT masses constantly. Some of the largest tech companies in Silicon Valley either specialize in virtualization or have entire divisions focused on offering virtualization alongside their other products. More and more infrastructure began converting to this system and I cannot go to a conference focusing on infrastructure for the cloud that doesn’t have presentations, classes or sessions on containers.Ĭontainers are the quintessential paradigm shift because virtualization has been around years and has been considered the gold standard for server and application hosting. Once the average infrastructure admin was able to create their own free container system and see the benefits, containerization caught on like wildfire. In a nutshell, containers optimize infrastructure server hosting with a much different approach than traditional virtualization allowing existing infrastructure to be used in a much more efficient way.Ĭontainers have been around for some time now, Google’s Kubernetes has been around for years, but in the last two years or so we’ve seen an absolute explosion in containerization thanks to startup rock stars like Docker. I love me some containers! If you’d like an excellent primer on containerization and how it differs from virtualization, I highly recommend you read Curtis Peterson’s excellent article on the subject. Containerization: Not Your Mama’s Virtual Server Previously, I wrote an article about why IT professionals may disagree with each other and one of the primary reasons is that many IT professionals may not be keeping up with more recent changes and approaches to how technology can be applied and secured.įollowing along these lines, here are a few major paradigm shifts technology has taken in recent years and why they are so important to the future of our computing experience. So, it’s in this vein that we consider how the technology industry is perpetually going through paradigm shifts with increasing frequency. We can almost reinvent ourselves time and again if we’re willing to be open to concepts and ideas. We believe one thing to be true, but with new information gathered and embraced, our view of the world can change to become a new reality. Paradigm shifts happen to most of us throughout our lives. If you use the self-service fountain inside the restaurant for your drink order, see the sign posted at the beverage fountain for beverage calories without ice. Calories for fountain beverages are based on standard fill levels plus ice. The nutrition information is based on standard product formulations and serving sizes. The nutrition information on this website is derived from testing conducted in accredited laboratories, published resources, or from information provided from McDonald's suppliers. Your daily values may be higher or lower depending on your calorie needs. ** Percent Daily Values (DV) are based on a 2,000 calorie diet. Percent Daily Values (DV) and RDIs are based on unrounded values. If you have questions about our food, please reach out to us directly using our contact us forms. We encourage our customers with food allergies or special dietary needs to visit for ingredient information, and to consult their doctor for questions regarding their diet. However, we also want you to know that despite taking precautions, normal kitchen operations may involve some shared cooking and preparation areas, equipment and utensils, and the possibility exists for your food items to come in contact with other food products, including allergens. Food and Drug Administration (eggs, dairy, wheat, soy, peanuts, tree nuts, fish, shellfish and sesame), so that our guests with food allergies can make informed food selections. As part of our commitment to you, we provide the most current ingredient information available from our food suppliers for the nine most common allergens as identified by the U.S. We understand that each of our customers has individual needs and considerations when choosing a place to eat or drink outside their home, especially those customers with food allergies. Last year, Melbourne's Swisse launched its wellness products in India.At McDonald's, we take great care to serve quality, great-tasting menu items to our customers each and every time they visit our restaurants. Last month, plant-based nutrition brand Oziva roped in Bollywood actor Deepika Padukone as its ambassador. Food delivering app Zomato has also announced its own functional food products line which will be available on its app. Several brands like TrueBasics, HealthKart, Wow Life Science, HealthVit, Himalaya, Inlife, and Biotrex have forayed into this space, both online and offline. The health supplement business space is growing in India with a study by Assocham and RNCOS estimating that it could reach $8.5 billion by 2022 from $2.8 billion in 2015. Fitday claims it made Rs 3 crore in the last financial year from the sales of Genomelabs products alone. The startup launched in April 2020 is funded by its parent Genomelabs. We have decided to run both avenues parallelly to reach more people," Raju told BusinessToday.In. We expect to double our revenue from our e-commerce platform. Hence, we have made this conscious decision to expand our stores. "We have done exceedingly well in the market since our inception and have had good traction on both online and offline platforms. The expansion plan comes at a time when the pandemic has wrecked offline businesses across sectors due to high operational costs amid falling footfalls, with the convenience and safety of ordering online taking over. 8/6/2023 0 Comments Tao venetian hotel(That doesn't even count its next-door sister, the 3,068-room Palazzo hotel.) The property is divided into two towers - the Venetian, with 4,027 rooms and the quieter 1,013-room Venezia, built in 2003 as an exclusive retreat for high-rolling gamblers. Opened in May 1999, the Venetian boasts more than 5,000 guest rooms, making it one of the biggest resorts in the country. Chic outdoor pools, renowned performances at the theater, and a large casino, however, are completely Vegas. Those willing to suspend their belief, if just for a moment - say, while strolling through a replica of Saint Mark’s Square - might feel they've stepped into Venice itself. The Italian city of Venice, of course, is the inspiration for the frescoed and gilded ceilings, gold paint, and serenading accordionists in the lobby next to the check-in desk. (Unlike the one in Venice, the Venetian's has a moving escalator floor, and leads to the Madame Tussaud’s Wax Museum.) There’s a "canal" where tourists can take a gondola ride, and a replica of the famous Rialto Bridge. It’s got all the trappings of a Las Vegas mega-resort - interactive elements, plenty of retail therapy, two towering guest room buildings, and a glam pool deck. It has a lot of great information.įinally, if you want to learn more about the city’s best dayclubs and beachclubs, visit our Dayclubs in Las Vegas page.The Venetian is the kind of place people either love or can’t stand. If you are looking for day and nightlife information in Vegas, what events you should go to, dress codes, and more, then check out our blog. Want to read more about our top partner venues? If you answered yes, then visit our Best Nightclubs In Las Vegas page. We’d love to walk you through the details! Want To Know More About Nightclubs And Dayclubs In Las Vegas? If you have questions about what you might need to pay and how you can increase your chances of getting in for free, call Vegas Good Life today at 70. Free Tao Beach Guest List – Call To Sign Up If you have a group of guys without an even male-to-female ratio, you may have to pay cover charges. The rules are a little different for groups of guys and gals. The perks of getting table service at TAO Beach Dayclub include (1) not paying cover charges, (2) getting VIP access to the beach club, i.e., no waiting in long lines, (3) having unlimited free mixers, (4) and having your own dedicated busser, waitress, and security staff. If you are concerned about the cost, we recommend reserving a table for a large group, as this will allow you to split the total amount among several people. Prices vary depending on the day and event. If you want table service, the cabanas and daybeds range in price from $1,500 to $6,000, although you may encounter prices outside of this range. If you are not a guest at the Venetian Hotel, you can, of course, still get access to TAO Beach via general admission, ordering a ticket in advance, or making a table service reservation. Note: If you are a Las Vegas local, then you can get into TAO Beach for free on regular weekdays, but you will be required to pay cover charges when the venue features special performers and has special events. Otherwise, you’ll have to pay a reduced cover charge. You will also have to pay for cocktails, beers, shots, and other kinds of drinks.Īnother thing to keep in mind is that you need to arrive before the guest list has closed. With that said, if you want to reserve a private area, such as one of the jungle cabanas, you will have to pay for it. You just have to be 21 years old or older and come dressed in the proper “pool attire” to get in. TAO Beach is complimentary for all Venetian guests, no matter what room you book inside the hotel. If you are a Venetian guest, then you can get in for FREE. The good news is, we have answers to your questions-whether you want confirmation about getting into TAO Beach Club for free, would like to learn how to get on their guest list, or want to know what their bottle service pricing is! Is Tao Beach Free For Venetian Guests? It can be confusing trying to find out the answer, especially if you are new to the city and are unfamiliar with how hotels here and their associated venues work. This is actually one of the more common questions we get given how popular the hotel is. For example, “Is Tao Beach free for Venetian guests?” People often have questions about what is and isn’t included in their hotel stay while in Vegas. 8/6/2023 0 Comments Breakaway clt 2022Information from the previous year may remain posted for certain festivals until updated plans are announced in particular, fees may change from year to year and dates may be estimated based on the previous festival. We try to update each weekend or when notified of new information. Need writing or editing help? Call on PC Nelson Communications today.ĭisclaimer: We've tried to gather the most up-to-date and accurate information available, but everything is subject to change and, for that matter, we and our sources make the occasional mistake. Search for your favorite band, festival or location (i.e., "Boone")Īsk us about the broad reach and low cost of advertising with Carolina Music Festivals. Spruce Pine BBQ Championship and Bluegrass Festival - Spruce Pineīascom Lamar Lunsford Festival - Mars Hillīalsam Range Art of Music Festival - Lake JunaluskaĬlick on the photos for pages about festivals outside North Carolina we have been to. Ol' Front Porch Music Festival - Oriental Hoppin' John Old-Time and Bluegrass Fiddlers' Convention - Silk HopeĮmerald Isle Beach Music Festival - Emerald Isle Stokes Stomp Festival on the Dan - Danbury Gears and Guitars Music Fests - Winston-SalemĬarolina Bible Camp Bluegrass Festival - Mocksville North Carolina Folk Festival - Greensboro John Coltrane International Jazz and Blues Festival - High PointĪntlers & Acorns: The Boone Songwriters Festival - Boone Happy Valley Fiddlers' Convention - LenoirĪfrican American Cultural Festival of Raleigh and Wake County - Raleigh McDowell County Country Music Roundup - MarionĮarl Scruggs Music Festival - Mill Spring PreddyFest Bluegrass Festival - Franklinton Mountain Dance and Folk Festival - Asheville Ocean City Jazz Festival - North Topsail BeachįabFest - Charlotte's Beatles Festival - CharlotteĪshe County Bluegrass and Old Time Fiddlers - Jefferson Surry Old Time Fiddlers Convention - Dobsonīrevard Summer Institute & Festival - Brevard Willow Oak Park Bluegrass Festival - Roxboroīoonerang Music and Arts Festival - Boone North Carolina Cigar Box Guitar Festival - Robbinsville Lil John's Mountain Music Festival - Snow Campīluegrass & Old-Time Fiddlers Convention - Mount AiryĬarolina Beach Music Festival - Carolina BeachĬarl Sandburg Songbag Festival - Flat Rock Malpass Brothers Country and Bluegrass Festival - DentonĪtlantic Beach Music Festival - Atlantic Beach North Carolina Brewers and Music Festival - Huntersville Shakori Hills Grassroots Festival of Music and Dance - Silk Hope Wub N Dub Arts and Music Festival - Willard Moo, Brew and 'Que Music Fest - Charlotte North Carolina 'Cuegrass Festival - Raleigh Singer and Songbirds Songwriter Festival - Statesville North Carolina HIP Music Festival - Triangleīlue, Brew and 'Que Festival - Kenansville North Carolina Jazz Festival - WilmingtonĪppalachian State Old-Time Fiddlers Convention - Boone If you know of a North Carolina music festival in January, please let us know! If we've left out any of your favorite North Carolina music festivals (or just some you know about), please let us know! If you haven't been to one because you have children, find a festival near you that you and the kids will enjoy. Most music festivals in North Carolina cater to families. Tweets by Stay up to date by following us as on, or, where we are Carolina Music Festivals. Ī photo with a festival listing indicates that we have more photos and an essay profiling that festival (like this one) click on the photo for more information. If you're interested in what's happening during a specific month, go to its page. Go to our master festival calendar below and click on an NC music festival's name for more information about that festival, including artist lineup, ticket prices, a map to the festival site and a link to the festival's website. In addition to the most up-to-date information available about dates, acts and prices, we'll tell you about the festival itself - the layout of the grounds, the best way to see it, what kind of vibe to expect (these additional pages are constantly under development as we make it to more festivals each season). The bands come and go, but the festivals play on.Ĭarolina Music Festivals is a comprehensive calendar and guide to multi-act, daylong or multiple-day music festivals in North Carolina. Just remind yourself of how great it’ll feel to trim the fat on your belongings, save money, possibly make some money, and start fresh in your new space. Real talk: Decluttering can be very emotional and overwhelming - especially if you’ve never done it before - so there’s no shame in needing a little motivation or inspiration to get prepared. Here are our best tips on getting the job done. The good news is it doesn’t have to be! Knowing when, where, and how to go through your things can help streamline the process, make it easier, and, dare we say, make it something you might even find a little fun. 11 Pro Tips To Help You Declutter Before MovingĮven with all the benefits, decluttering can feel like a daunting task to undertake, particularly when paired with a move. Looking for top tips on how to stay on track, on time, and feeling good through the decluttering process? You’ve come to the right place! We’ve teamed up with Maija Diethelm, a professional organizer with more than a decade of experience decluttering spaces, to share our best tips on going through your belongings so you can start fresh in your new place. Plus, if you donate or host a garage or estate sale while downsizing, you can even pad your pockets with a little extra cash in the process. Why? The more stuff you have, the more it costs to move it. Not only can a large declutter before moving save you time and energy on both ends of your move it can also help save you money. When’s the last time you did some decluttering? If you’re planning a move, you might want to consider taking the plunge to pare down your belongings. |
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